It is still not clear if you are actually transmitting a carrier or not. It may not matter if the radio shows a full red line or not. If you key the radio, can you hear an audio-less signal (carrier) on another radio? if not, then you are not actually transmitting, and mic gain really does not enter into whether or not you are hitting a repeater, since it is the carrier that will activate the repeater, even if you have no audio. If another radio can 'hear' your carrier, but no audio when you talk, then the mic may be defective.
Another thought: Does the repeater you are trying to access have a CTCSS (PL) tone needed by your transmitter, and if so, are you sure you have it programmed in correctly?